RLC Wijesundera, SSBDP Soysa, ED de Silva, CM Nanayakkara\r\n
Muthuthanthriege Dilusha Maduranganie Fernando, Antioxidant potential and content of the polyphenolic secondary metabolites of white rot macrofungi; Flavodon flavus (Klotzsch.) and Xylaria feejeensis (Berk.)(2016)SDRP Journal Of Plant Science 1(1)
White rot fungi are known to possess an efficient antioxidative system consisting of enzymatic elements and polyphenolic secondary metabolites which is implicated in the decay process of secondary cell wall in the wood. The current study was aimed at determining the antioxidant capacity, the contents of phenolic and flavonoid metabolites of two white rot macrofungi; Flavodon flavus (Klotzsch.) and Xylaria feejeensis (Berk.) collected from Dambulla and Mahiyanganaya in Sri Lanka. Crude extracts of macrofungi were prepared using methanol and dichloromethane by sonication extraction. Antioxidant capacity of the crude extracts was investigated using 1,1-diphenyl-2-picrylhydrazyl scavenging method using ascorbic acid as the standard antioxidant agent. The quantity of phenolic compounds was determined by Folin-Ciocalteau spectrophotometric method. Flavonoid contents of white rot fungi were evaluated by the aluminum chloride colorimetric method. F. flavus and X. feejeensis showed potent antioxidant activity with EC50 values of 77.00 ± 0.18 µg/ml and 98.4 ± 0.28 µg/ml respectively. Further, both species showed high levels of total phenol and flavonoid contents. In conclusion, these findings suggest studied white rot fungi have strong antioxidative systems and imply that phenol and flavonoid compounds possibly contribute to the observed promising antioxidant activity.
Key words: White rot fungi, Antioxidant activity, EC50, Phenol content, Flavonoid content
Adebayo, E.A. Oloke, J.K. Ayandele, A.A. Adegunlola. C.O. 2012. Phytochemical, antioxidant and antimicrobial assay of mushroom metabolite from Pleurotus pulmonarius. J. Microbiol. Biotech. Res., 2 (2): 366-374.
Barros, L. Falc?o, S. Baptista, P. Freire, C. Vilas-Boas, M. Ferreira, I.C.F.R. 2008. Antioxidant activity of Agaricus sp. mushrooms by chemical, biochemical and electrochemical assays. J. Food Chem., 111: 61?66.View Article
Baskar, R. Lavanya, R. Mayilvizhi, S. Rajasekaran, P. 2008. Free radical scavenging activity of antitumour polysaccharide fractions isolated from Ganoderma lucidum (Fr.) P. karst. J Natur prod radiance., 7(4): 320-325.
Blanchette, R.A. Burnes, T.A. Leatham, G.F. Effland, M.J. 1988. Selection of White-rot Fungi for Biopulping. Biomass., 15 : 93-101. 90099-6View Article
Brand-Williams, W. Cuvelier, M.E. Berset, C. 1995. Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technology., 28: 25-30. 80008-5View Article
Bray, H.G. and Thrope, W.V. 1954. Analysis of phenolic compounds of interest in metabolism. Meth. Biochem. Anal., 1: 27-52. PMid:13193524View Article PubMed/NCBI
Chang, C. Yang, M. Wen, H. Chern, J. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food and Drug Anal., 10:178-182.
Chang, H. Ho, Y. Sheu, M. Lin, Y. Tseng, M. Wu, S. Huang, G. Chang, Y. 2007. Antioxidant and free radical scavenging activities of Phellinus merrillii extracts. Bot Stud., 48: 407-417.
Diaz, P. Jeong, S.C. Lee, S. Khoo, C. Koyyalamudi, S.R. 2012. Antioxidant and anti-inflammatory activities of selected medicinal plants and fungi containing phenolic and flavonoid compounds. Chinese Medicine., 7: 26-35. PMid:23176585 PMCid:PMC3577437View Article PubMed/NCBI
Fernando, M.D.M., Wijesundera R.L.C., Soysa S.S.B.D.P., de Silva E.D., Nanayakkara C.M., (2015). In vitro cytotoxicity and antioxidant activity of the Sri Lankan Basidiomycete, Anthracophyllum lateritium. BMC Complementary and Alternative Medicine. 15: 398-407. DOI 10.1186/s12906-015-0924-9.View Article
Folin, O. Ciocalteu, V. 1927. On tyrosine and tryptophan determination in proteins. J Biol and Chem., 27: 627-650.
Jaszek, M. OsiNska-Jaroszuk, M. Janusz, G. Matuszewska, A. Stefaniuk, D. Sulej, J. Polak, J. Ruminowicz, M. Grzywnowicz, K. Jarosz-WilkoBazka, A. 2013. New bioactive fungal molecules with high antioxidant and antimicrobial capacity isolated from Cerrena unicolor idiophasic Cultures. BioMed Research International., 2013: 1-11. PMid:23936810 PMCid:PMC3727119View Article PubMed/NCBI
Karunarathna, S.C. Udayanga, D. Maharachchikumbura, S.N. Pilkington, M. Manamgoda, D.S. Wijayawardene, D.N.N. Ariyawansa, H.A. Bandara, A.R. Chukeatirote, E. McKenzie, E.H.C. Hyde, K.D. 2012. Current status of knowledge of Sri Lankan mycota. Current Research in Environmental & Applied Mycology., 2(1):18-29.
Keles, A. Koca, I. Gen?celep, H. 2011. Antioxidant properties of wild edible mushrooms. J Food Process Technol., 2:1-6.
Menikpurage, I.P. Soysa, S.S.S.B.D.P. Abeytunga, D.T.U. (2012). Antioxidant activity and cytotoxicity of the edible mushroom, Pleurotus cystidiosus against Hep-2 carcinoma cells. J NSF, 40(2):107-114.View Article
Nahata, A. (2013). Ganoderma lucidum: A Potent Medicinal Mushroom with Numerous Health Benefits Pharmaceut Anal Acta, 4(10): 1000e159.
Raghukumar, C. Rivonkar, G. 2001. Decolorization of molasses spent wash by the white-rot fungus Flavodon flavus, isolated from a marine habitat. Applied Microbiol and Biotech., 55(4), 510-514. PMid:11398935View Article PubMed/NCBI
Ram?rez-Anguiano, A.C. Santoyo, S. Reglero, G. Soler-Rivas, C. 2007. Radical scavenging activities, endogenous oxidative enzymes and total phenols in edible mushrooms commonly consumed in Europe. J Sci Food Agric., 87: 2272?2278.View Article
Ravipati, A.S. Zhang, L. Koyyalamudi, S.R. Jeong, S.C. Reddy, N. Bartlett, J. Smith, P.T. Shanmugam, K. M?nch. G, Wu, M.J. Satyanarayanan, M. Vysetti, B. 2012. Antioxidant and anti-inflammatory activities of selected Chinese medicinal plants and their relation with antioxidant content. BMC Comp and Alter Med., 12:173-187.View Article
Rawat, A. Mohsin, M. Negi, PS. Sah, AN. Singh, S. 2013. Evaluation of polyphenolic contents and antioxidant activity of wildly collected Ganoderma lucidum from central Himalayan hills of India. Asian J Plant Sci Res., 3(3): 85-90.
Samarakoon, K. Lee, j. De Silva, E.D. Kim, E. Wijesundara, R.L.C. Lakmal, H.H.C. Jeon, Y. 2013. Bioactivity evaluation of organic solvent extractions of Ganoderma lucidum: a Sri Lankan basidiomycete. J.Natn.Sci.Foundation Sri Lanka., 41 (3): 249-257.View Article