Anupama Sharma, Nivedita Sharma, Jasveen Bajwa
jasveen bajwa, Evaluation of Enterococcus faecium Ch-1 isolated from Chuli- A traditional fermented apricot product of Trans Himalayan region(2017)SDRP Journal of Food Science & Technology 2(1)
Background: Chuli is a naturally fermented apricot fruit product of Himachal Pradesh and is a rich source of polyphenols and other phytochemicals such as betacarotene and ascorbic acid. In the present research work potential lactic acid bacteria were isolated and explored for its novelty as potential probiotics.
Methods: Isolates were screened on the basis of broadest inhibitory spectrum against various food borne pathogens i.e , and hence was selected for further study i.e acid and bile tolerance, adherence to gastric mucin sensitivity towards different antibiotics. its relative safety as probiotic candidate was also assessed.
Results: Highest antagonism was show by Enterococcus faecium Ch-1 and was found to be tolerant to low pH and high bile concentrations, adherance to gastric mucin. E. faecium Ch-1 was found negative for gelatinase, DNase enzyme activity and haemolysis thus validating its relative safety as probiotic candidate.
Conclusion: Enterococcus faecium Ch-1 was found to be a good probiotic strain with cumulative probiotic score of 100% therefore, could be promising for the development as suitable isolate for use in functional foods.
Key words: acid and bile tolerance, antagonism, antibiotics, chuli, DNase, functional foods, gastric mucin , gelatinase, haemolysis, probiotic.
Pun LH and Mares V. 2000. The sustainable development of mountain regions. A paradigm shift and new considerations. In: contribution of livestock to mountain livelihoods: Research and development issues. ICIMOD (International center for integrated mountain development) , Kathmandu, Nepal. Pp 35-36
Gautam N and Sharma N. 2014. Evaluation of probiotic potential of new bacterial strain L. spicheri G2 isolated from Gundruk. Proceedings of the National Academy of Sciences India. Sect. B. Biological Sciences.
Nueno PC and Narbad A. 2011. Probiotic assessment of Enterococcus faecalis CP58 isolated from human gut. International Journal of Food Microbiology 145, 300-394.
Ahmadova A. Todorov SD, Choiset Y, Rabesona H, Zadi TM, Kuliyev A, Franco BD Chobert JM and Haertle T. 2013. Evaluation of antimicrobial activity, probiotic properties and safety of wild strain Enterococcus faecium AQ71 isolated from Azerbaijani Motal cheese. Food Control, 30, 631?641.View Article
Vahjen W, Taras D, and Simon O. 2015. Effect of the Probiotic Enterococcus faecium NCIMB10415 on Cell Numbers of Total Enterococcus spp., E. faecium and E. faecalis in the Intestine of Piglets. Curr. Issuea Intestinal Micobiol. 8: 1-8.
Murray BE. 1990. The life and times of the Enterococcus. Clinical Microbiology Reviews 3, 46?65. PMid:2404568 PMCid:PMC358140View Article PubMed/NCBI
Barbosa J, Borges S and Teixeira P. 2014. Selection of potential probiotic Enterococcus faecium isolated from Portuguese fermented food. International Journal of Food Microbiology 191, 144-148. PMid:25268323View Article PubMed/NCBI
Hlivak P, Odraska J, Ferencik M, Ebringer L, Jahnova E and Mikes A. (2005). One-year application of probiotic strain Enterococcus faecium M-74 decreases serum cholesterol levels. Bratisl. Lek. Listy 106, 67?72.
Huang Y and Zheng Y. 2009. The probiotic Lactobacillus acidophilus reduces cholesterol absorption through the down-regulation of Niemann?Pick C1-like 1 in Caco-2 cells. British Journal of Nutrition 9, 1?6.
Pascual L, Ruiz F, Giordano W and Barberis I L. 2010. Vaginal colonization and activity of the probiotic bacterium Lactobacillus fermentum L23 in a murine model of vaginal tract infection. Journal of Medical Microbiology 59, 360?364. PMid:19926731View Article PubMed/NCBI
Salminem S, Isolauri E and Salminem E. 1996. Clinical uses of probiotics for stabilizing the gut mucosal barrier: successful strains and future challenges. Antonie van Leeuwenhoek 70, 347?358.View Article
Gupta A and Sharma N. 2017. Probiotic Potential of Lactic Acid Bacteria Ch-2 Isolated from Chuli Characterization of Potential Probiotic Lactic Acid Bacteria- Pediococcus acidilactici Ch-2 Isolated from Chuli- A Traditional Apricot Product of Himalayan Region for the Production of Novel Bioactive Compounds with Special Therapeutic Properties. Journal of Food: Microbiology, Safety and Hygiene. 2:1.View Article
Lebeer S, Verhoeven T L A, Perea V M Vanderleyden J and Keersmaecker D. 2007 Impact of environmental and genetic factors on biofilm formation by the probiotic strain Lactobacillus rhamnosus GG. Appl Environ Microbiol 73: 6768?6775. PMid:17827316 PMCid:PMC2074970View Article PubMed/NCBI
Bao Y, Zhang Y, Zhang Y, Liu Y, Wang S, Dong X, and Zhang H. 2010. Screening of potential probiotic properties of Lactobacillus fermentum isolated from traditional dairy products. Food Control 21, 695?701. doi:10.1016/j.foodcont.2009.10.010.View Article
De M, Rogosa J and Sharpe M. 1960. A medium for the cultivation of lactobacilli. Journal of Applied Bacteriology 3, 13-135.
Barefoot S F, Klaenhammer T R. 1983. Detection and activity of Lactacin B, a bacteriocin produced by Lactobacillus acidophilus. Applied and Environmental Microbiology 45(6), 1808-1815. PMid:6410990 PMCid:PMC242543
Kimura H, Sashihara T, Matsusaki H, Sonomoto K and Ishizaki A. 1998. Novel bacteriocin of Pediococcus sp. ISK-1 isolated from well ? aged bed of fermented rice bran. Annals of New York Academy of Science 864: 345-348. PMid:9928112View Article PubMed/NCBI
Thirabunyanon M, Boonprasom P and Niamsup P. 2009. Probiotic potential of lactic acid bacteria isolated from fermented dairy milks on antiproliferation of colon cancer cells. Biotechnology Letters 31: 571?576. PMid:19116692View Article PubMed/NCBI
Hargrove RE and Alford J A. 1978. Growth rate and feed efficiency of rats fed yogurt and other fermented milks. J. Dairy Sci., 61: 11-19. 83544-9View Article
Gupta H, Malik R K. 2007. Incidence of virulence in bacteriocin ?producing enterococcal isolates. INRA, EDP Sciences. 587-601.
Harrigan W F, and Cance M E.(1990). Laboratory Methods in Food and Dairy Microbiology. Academic Press, London. PMCid:PMC1452225
Liong M T and Shah N P. 2004. Acid and bile tolerance and cholesterol removal ability of Lactobacilli strains. Journal of Dairy Science 88: 55-56. 72662-XView Article
Gilliland SE and Walker D K. 1990. Factors to consider when selecting a culture of L. acidophilus as a dietary adjunct to produce a hypercholesterolemic effect in humans. Journal of Dairy Science 73, 905-909. 78747-4View Article
Zhang B, Wang Y, Tan Z, Li Z, Jia Z and Huang Q. 2016. Screening of probiotic activities of lactobacilli strains isolated from traditional Tibetan qula, a raw yak milk cheese. Asian Australas. J. anim.Sci. 10: 1490-1499 PMid:26954218 PMCid:PMC5003976View Article PubMed/NCBI
Del R B, Sgorbati, B., Miglioli, M., & Palenzona, D. (2000). Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum. Letters in Applied Microbiology 31, 438-442.View Article
Handley PS, Harty DWS, Wyatt JE, Brown CR, Doran JP, Gibbs ACC. A comparison of the adhesion, coaggregation and cell-surface hydrophobicity propertiesof fibrillar and fimbriate strains of Streptococcus salivarius. J Gen Microbiol. 1987; 133: 3207-3217. PMid:2895798
Rosenberg, M., Gutnick, D., & Rosenberg, E. (1980). Adherence of bacteria to hydrocarbons: a simple method for measuring cell surface hydrophobicity. FEMS Microbiology Letters 9, 29-33.View Article
Styriak, I., Nemcova, R., Chang, Y.H., & Ljungh, A. (2003). Binding of extracellular matrix molecules by probiotic bacteria. Letters in Applied Microbiology 37, 329?333. PMid:12969498View Article PubMed/NCBI
Collado, M.C., Gueimonde, M., Hernandez, M., Sanz, Y., & Salminen, S. (2005). Adhesion of selected Bifidobacterium strains to human intestinal mucus and its role in enteropathogen exclusion. Journal of Food Protection 68(12): 2672?2678. PMid:16355841View Article PubMed/NCBI
Coppola G, Vandenheede M, Clemente LD, Ambrosini A, Fumal A, De Pasqua V, Schoenen J. Somatosensory evoked high-frequency oscillations reflecting thalamocortical activity are decreased in migraine patients between attacks. Brain 2005; 128: 98?103. PMid:15563513View Article PubMed/NCBI
Guodong Zhou, Jian Su, Jie Zhang, and Min Zhang. 2005. Exploring various knowledge in relation extraction. In ACL-05, pages 427?434, Ann Arbor, MI.
Saelim, K., Sohsomboon, N., Kaewsuwan, S., & Maneerat, S. (2012). Probiotic properties of Enterococcus faecium CE5-1 producing a bacteriocin-like substance and its antagonistic effect against antibiotic-resistant enterococci in vitro. Czech Journal of Animal Science 57(11), 529?539.
Botes, M., van Reenen, C.A., & Dicks, L.M.T. (2008). Evaluation of Enterococcus mundtii ST4SA and Lactobacillus plantarum 423 as probiotics by using a gastro-intestinal model with infant milk formulations as substrate. International Journal of Food Microbiology 128, 362?370. PMid:18963159View Article PubMed/NCBI
Gunn, J.S. (2000). Mechanisms of bacterial resistance and response to bile. Microbes and Infection 2, 907-913. 00392-0View Article
Abbasiliasi, S., Tan, J.S., Ibrahim, T.A.T., Ramanan, R.N., Vakhshiteh, F., Mustafa, S., Ling, T.C., Rahim, R.A., & Ariff, A.B. (2012). Isolation of Pediococcus acidilactici Kp10 with ability to secret bacteriocin-like inhibitory substance from milk products for applications in food industry. BMC Microbiology, 12, 260 PMid:23153191 PMCid:PMC3571982View Article PubMed/NCBI
Abdhul, K., Ganesh, M., Shanmughapriya, S., Kanagavel, M., Anbarasu, K., & Natrajaseenivasan, K., 2014. Antioxidant activity of exopolysaccharide from probiotic strain Enterococcus faecium (BDU7) from Ngari. International Journal of Biological Macromolecules 70, 450-454. PMid:25062992View Article PubMed/NCBI
Todorov, S.D., von Mollendorff, J. W., Moelich, E., Muller, N., Witthuhn, R.C., & Dicks, L.M.T. (2009). Evaluation of Potential Probiotic Properties of Enterococcus mundtii, Its Survival in Boza and in situ Bacteriocin Production. Food Technology Biotechnology 47(2), 178-191.
Kos B., Suskovic J., Vukovic S., Simpraga M., Frece J. and Matosic S. (2003). Adhesion and aggregation ability of probiotic strain lactobacillus acidophilus M92. Journal of Applied Microbiology. 94: 981-987 PMid:12752805View Article PubMed/NCBI
Tareb, R., Bernaedeau, M., Gueguen, M., & Vernoux, J.P. (2013). In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with food borne zoonotic bacteria, especially Campylobacter jejuni. Journal of Medical Microbiology 62, 637-649. PMid:23329323View Article PubMed/NCBI
Tuomola, E., Crittenden, R., Playne, M., Isolauri, E. and Salminen, S. (2001) Quality assurance criteria for probiotic bacteria. Am J Clin Nutr 73, 393?398.
Araujo, T. F., & Ferreira, C.L.L.F. (2013). The genus Enterococcus as probiotics: Safety concerns. Brazilian Archives of Biology and Technology 56(3), 457-466.View Article
Kainulainen, V., Tang, Y., Spillmann, T., Kilpinen, S., Reunanen, J., Saris, P.E.J., & Satokari, R. (2015). The canine isolate Lactobacillus acidophilus LAB20 adheres to intestinal epithelium and attenuates LPS-induced IL-8 secretion of enterocytes in vitro. BMC Microbiology 15, 4 DOI 10.1186/s12866-014-0337-9. PMid:25591990 PMCid:PMC4320479View Article PubMed/NCBI