SDRP Journal of Earth Sciences & Environmental Studies

HYDROCARBON-DEGRADING BACTERIA IN PETROLEUM-CONTAMINATED SOIL AT SUAME MAGAZINE

Co-Authors

Mak-Mensah Ephraim, Mills-Robertson Felix Charles

Citation

Agyeman-Duah Eric, HYDROCARBON-DEGRADING BACTERIA IN PETROLEUM-CONTAMINATED SOIL AT SUAME MAGAZINE(2017)SDRP Journal of Earth Sciences & Environmental Studies 2(3)

Abstract

Accidental oil spill has been a global problem especially the oil producing developing countries.  Bioremediation remains the best and safest option for eradicating these toxic substances from the environment.  In this study, petroleum hydrocarbon-degrading bacteria associated with crude oil contaminated soils were investigated.  Samples were taken from 3 different garages at 3 different soil depths (5, 10 and 15 cm) in Suame, a suburb of Kumasi and analyzed microbiologically.  Colonies were isolated and cultured on nutrient agar covered with used engine oil to check for their hydrocarbon-degrading ability.  The enumeration of the microbes for sites A, B and C were 1.695 × 107, 1.817 × 106 and 1.511 × 105 CFU/g respectively.  Pseudomonas spp., Acinetobacterium spp., Bacillus spp., Aeromonas spp. and Micrococcus spp. were found to possess hydrocarbon-degrading ability.  Thus, in this study, bacteria with hydrocarbon-degrading ability were found to inhabit engine oil-contaminated soils at Suame magazine in Kumasi, Ghana.

 

Key words: Petroleum hydrocarbons, Pseudomonas spp., Acinetobacterium spp., Bacillus spp., Micrococcus spp.

References

  1. Lang X, Dalai A, Bakhshi N, Reaney M, Hertz P. 2001. Preparation and characterization of bio-diesels from various bio-oils. Bioresource Technology, 80: 53-62. 00051-7

    View Article           

  2. Venosa AD. 2004. Literature review on the use of commercial bioremediation agents for cleanup of oil-contaminated estuarine environments. University of Cincinnati, U.S.A.

  3. Caravanos J, Clarke EE, Osei CS, Amoyaw-Osei Y. 2013. Exploratory Health Assessment of Chemical Exposures at E-Waste Recycling and Scrapyard Facility in Ghana. Journal of Health and Pollution, 3(4): 11-22.

    View Article           

  4. Achawa O, Danso-Boateng E. 2013. Environmental Management in the Oil, Gas and related Energy Industries in Ghana. International Journal, 4(2): 117-122.

  5. Thamer M, Al-Kubaisi AR, Zahraw Z, Abdullah HA, Hindy I, Khadium AA. 2013. Biodegradation of Kirkuk light crude oil by Bacillus thuringiensis, Northern of Iraq. Natural Science, 5(7): 865-873.

    View Article           

  6. Sonobe T, Mano Y, Akoten J, Otsuka K. 2010. Assessing the Impacts of Management Skill Training in Ghana and Kenya. CSAE Economic Development in Africa Conference, 2010: 21-23. PMCid:PMC3023525

  7. Adeya N. 2006. Knowledge, Technology and Growth: The Case Study of Suame Manufacturing Enterprise Cluster in Ghana. WBI Cluster Case Study, pp. 1-12.

  8. Adeya, C. N. 2008. The Suame Manufacturing Cluster in Ghana. Knowledge, Technology, and Cluster-based Growth in Africa, 15: 1-12.

  9. Iddrisu A, Mano Y, Sonobe T. 2012. Entrepreneurial skills and industrial development: The case of a car repair and metalworking cluster in Ghana. Journal of the Knowledge Economy, 3: 302-326.

    View Article           

  10. Adade-Boateng, D. (2012). Rhizoremediation Of Hydrocarbon Contaminated Soils: A Case Study At Newmont Ghana Gold Limited (Nggl) Ahafo- Kenyasi. KNUST.

  11. Megharaj M, Ramakrishnan B, Venkateswarlu K, Sethunathan N, Naidu R. 2011. Bioremediation approaches for organic pollutants: a critical perspective. Environment International, 37: 1362-1375. PMid:21722961

    View Article      PubMed/NCBI     

  12. Bose P. 2010. Gram Negative Bacteria Identification. BIOL, 3444: 219-226

  13. Fierer N, Schimel NP, Holden PA. 2003. Variations in microbial community and composition through two soil depth profiles. Soil Biology and Biochemistry, 351(1): 167-176. 00251-1

    View Article           

  14. Hamza A, Rabu A, Amzy RFH, Yussoff NA. 2010. Isolation and characterization of bacteria degrading Sumandak and South Angsi oils. Sains Malaysia, 39(2): 161-168.

  15. Latha R, Kalaivani R. 2011. Bacterial Degradation of Crude Oil by Gravimetric Analysis. Advances in Applied Science Research, 3(5): 2789-2795.

  16. Beveridge TJ. 2001. Use of the Gram stain in microbiology. Biotechnic and Histochemistry, 76: 111-118 PMid:11475313

    View Article      PubMed/NCBI     

  17. Chester B, Moskowitz LB. 1987. Rapid catalase supplemental test for identification of members of the family Enterobacteriaceae. Journal of clinical microbiology, 25: 439-441. PMid:3818934 PMCid:PMC265920

  18. Dongfeng Z, Weilin W, Yunbo Z, Qiyou L, Haibin Y, Chaocheng Z. 2011. Study on Isolation, Identification of A Petroleum Hydrocarbon-degrading Bacterium Bacillus fusiformis sp. and Influence of Environmental Factors on Degradation Efficiency. China Petroleum Processing and Petrochemical Technology, 13: 74-82.

  19. Marwah T, Abdul Rahman AK, Zahraa Z, Hussam Aldin A, Iman H, Ammar Abd K. 2013. Biodegradation of Kirkuk light crude oil by Bacillus thuringiensis, Northern of Iraq. Natural Science, 5: 865.

    View Article           

  20. Ameen IA, Danjuma BY, Raji OY. 2006. Isolation And Identification Of Hydrocarbon-degrading Micro-Organisms From Crude Oil Contaminated Soils. Advances in Applied Science Research, 3(5): 2789-2795.

  21. Panda S, Kar R, Panda C. 2013. Isolation and identification of petroleum hydrocarbon-degrading microorganisms from oil contaminated environment. International Journal Of Environmental Sciences, 3(5): 1314-1321.

  22. Erdo?an EE, ?ahin F, Karaca A. 2012. Determination of petroleum-degrading bacteria isolate of rom crude oil-contaminated soil in Turkey. African Journal of Biotechnology, 11: 4853-4859.

  23. Sneath PH, Mair NS, Sharpe ME, Holt JG. 1986. Bergey's manual of systematic bacteriology, 2: 1-8.

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