Journal of Chemical Engineering And Bioanalytical Chemistry

Phytochemical analysis and antioxidant activity of chromatographic fractions from Eucalyptus globulus plant

Co-Authors

Zakia Bey-Ould Si Said, Hayat Idir- Himed, Hocine Remini, Sakina Slimani, Jean-Paul Mazauric, Khodir Madani, Lila Boulekbache-Makhlouf

Citation

BOULEKBACHE LILA, Phytochemical analysis and antioxidant activity of chromatographic fractions from Eucalyptus globulus plant(2016)Journal of Chemical Engineering And Bioanalytical Chemistry 1(1)

Abstract

Extracts from crude and chromatographic fractions from fruit and leaves of Eucalyptus globulus (E. globulus) plant, were screened for their phenolic compounds and antioxidant activity. The amount of phenolic compounds in the crude extracts was higher in the fruit part excepted for flavonoid compounds. The fractions from different parts of the plant were found to contain differents levels of total phenolic and tannin contents, and fraction b of fruit and leaves extracts is the richest one (178.20±2.14 mg gallic acid equivalent (GAE)/g of crude extract (CE); 86.12±1.51 mg tannic acid equivalent (TAE)/g CE and 206.41±1.52 mg GAE/g CE; 55.81±0.96 mg TAE/g CE, respectively). Crude extract of fruits shows a best reducing power, scavenging activity against 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical and hydrogen peroxide (H2O2). Among leaves extracts, fraction c exhibit the better reducing power and DPPH scavenging capacity, whereas fraction b, was the most active in H2O2 assay. The observed results which correlate positively with phenolic contents plead in favour of the use of crude extract of E. globulus fruits as potential natural antioxidant in addition to leaves which have been reported to be used as natural food additive.

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