SDRP Journal of Earth Sciences & Environmental Studies



Mak-Mensah Ephraim, Mills-Robertson Felix Charles




Accidental oil spill has been a global problem especially the oil producing developing countries.  Bioremediation remains the best and safest option for eradicating these toxic substances from the environment.  In this study, petroleum hydrocarbon-degrading bacteria associated with crude oil contaminated soils were investigated.  Samples were taken from 3 different garages at 3 different soil depths (5, 10 and 15 cm) in Suame, a suburb of Kumasi and analyzed microbiologically.  Colonies were isolated and cultured on nutrient agar covered with used engine oil to check for their hydrocarbon-degrading ability.  The enumeration of the microbes for sites A, B and C were 1.695 × 107, 1.817 × 106 and 1.511 × 105 CFU/g respectively.  Pseudomonas spp., Acinetobacterium spp., Bacillus spp., Aeromonas spp. and Micrococcus spp. were found to possess hydrocarbon-degrading ability.  Thus, in this study, bacteria with hydrocarbon-degrading ability were found to inhabit engine oil-contaminated soils at Suame magazine in Kumasi, Ghana.


Key words: Petroleum hydrocarbons, Pseudomonas spp., Acinetobacterium spp., Bacillus spp., Micrococcus spp.


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