Honghong Wu, Chiyu Zhang and Yihong Hu
Yihong Hu, Nucleic Acid Sequence-Based Amplification in Diagnosis of Disease Pathogens(2017)Journal Of Advances In Allergy & Immunologic Diseases 1(1)
Nucleic acid sequence-based amplification (NASBA) is a simple technology which is used to amplify nucleic acid isothermally. The template is mainly RNA, can also be DNA. Combined with molecular beacon assay, NASBA is widely used to diagnose virus, bacteria, fungus, protozoa and other pathogens. NASBA has comparable sensitivity and specificity to PCR, but it’s complicated to be applicable as it could not be integrated efficiently with relevant detection techniques. Here, we summarize the development of NASBA, especially its application in infectious disease diagnosis based on previous study, which provide reference for developing NASBA technology to detect trace amount of disease pathogens.