Huang J(1), Zhuo Y(2), Lu J(3), Lai Q(3), Zhang Y(3). Author information:
(1)Engineering Technological Center of Mushroom Industry, Minnan Normal
University, Zhangzhou, Fujian, 363000, China; Collaborative Innovation Center of
Mushroom Health Industry, Minnan Normal University, Zhangzhou, Fujian, 363000,
China; School of Life Sciences & Biotechnology College, Minnan Normal
University, Zhangzhou, Fujian, 363000, China. Electronic address:
(2)Zhangzhou No.3 Middle School, Zhangzhou, Fujian, 363000, China.
(3)School of Life Sciences & Biotechnology College, Minnan Normal University,
Zhangzhou, Fujian, 363000, China.
During the Agaricus bisporus canning processes, a large number of water-soluble elements were dissolved into the processing hot water. This study was conducted to use the industrial wastewater of A. bisporus to prepare agricultural microbial fertilizer. In the work, the influence of 6 different liquid fermentation factors on the total biomass of living Bacillus cereus was evaluated with the one-factor-at-a-time method and the Plackett-Burman design. The total biomass of living B. cereus was most influenced by fermentation temperature, shaking speed, and inoculation volume, which were identified as the most critical independent variables for the B. cereus biomass. The approximate ranges of optimal fermentation conditions for the three key factors were identified by the path of steepest ascent. The center point of these factors were 24 ℃ of temperature, 250 rpm of shaking speed and 12 % inoculum amount, respectively. The Box-Behnken design was applied to derive a statistical model for optimizing the three fermentation factors for B. cereus biomass. After further optimizations based on statistical predictions, the optimum fermentation parameters for B. cereus cultured in the A. bisporus industrial wastewater were fermentation temperature of 24.8 °C, shaking speed of 234 rpm, inoculum dose of 12.2 % (v:v, %), industrial wastewater concentration of 4%, initial pH values of 6.5, loading liquid of 60 mL/250 mL, and culture time of 24 h. Culturing with the optimal fermentation conditions resulted in the biomass of B. cereus of 1.35 ± 0.02 × 109 Obj/mL (N = 3), which was consistent with the predicted values (1.32 × 109 Obj/mL) predicted by the corresponding regression models (p < 0.05), and more, was also far higher than that of the standard of agricultural bacterial fertilizers in People's Republic of China. Further, the results of field trial indicated that the of B. cereus liquid fertilizer can remarkably enhance the yield of Brassica chinensis L. It is practicable to make use of the industrial wastewater of A. bisporus to prepare the microbial fertilizer.
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