CRISPR/Cas Powered Multiplexed Biosensing.


University of Freiburg, Department of Microsystems Engineering (IMTEK), Laboratory for Sensors, 79110 Freiburg, Germany; University of Freiburg, Freiburg Center for Interactive Materials and Bioinspired Technologies (FIT), 79110 Freiburg, Germany; Imperial College London, Department of Bioengineering, Royal School of Mines, SW7 2AZ, London, UK. Electronic address: [Email]


Multiplexed CRISPR/Cas biosensing offers various possibilities for diagnosing different diseases by analyzing many analytes from one single specimen. However, in this relatively new field, nearly no multiplexing approaches exist, as many challenges need to be addressed. We discuss the reasons behind it and possible strategies to push the multiplexing level.


CRISPR/Cas systems,multiplexing,nucleic acid diagnostics,point-of-care testing,