Ma H(1), Zhang X(1), Yang Y(2), Li S(1), Huo J(1), Liu Y(1), Guan M(1), Zhen M(1), Shu C(1), Li J(1), Wang C(1). Author information:
(1)Beijing National Research Center for Molecular Sciences, Key Laboratory of
Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese
Academy of Science, Beijing 100190, China.
(2)Department of General Surgery, The Fourth Hospital of Hebei Medical
University, Shijiazhuang 050011, China.
Hydrophilic fullerene derivatives get notable performance in various biological applications, especially in cancer therapy and antioxidation. The biological behaviors of functional fullerenes are much dependent on their surface physicochemical properties. The excellent reactive oxygen species-scavenging capabilities of functional fullerenes promote their outstanding performances in inhibiting pathological symptoms associated with oxidative stress, including neurodegenerative diseases, cardiovascular diseases, acute and chronic kidney disease, and diabetes. Herein, fullerene derivatives with reversed surface charges in aqueous solutions are prepared: cationic C60-EDA and anionic C60-(EDA-EA). Under the driving force of membrane potential (negative inside) in the cell and mitochondria, C60-EDA is much rapidly taken in by cells and transported into mitochondria compared with C60-(EDA-EA) that is enriched in lysosomes. With high cellular uptake and mitochondrial enrichment, C60-EDA exhibits stronger antioxidation capabilities in vitro than C60-(EDA-EA), indicating its better performance in the therapy of oxidation-induced diseases. It is revealed that the cellular uptake rate, subcellular location, and intracellular antioxidation behavior of fullerene derivatives are primarily mediated by their surface charges, providing new strategies for the design of fullerene drugs and their biological applications.
OUR JOURNALS
Having over 250 Research scholars worldwide and more than 400 articles online with open access.
