Chemical constituents and biological activities of the fruits of Knema pachycarpa de Wilde.


Giap TH(1), Duc PM(2), Van The N(3), Popova M(4), Bankova V(4), Hue CT(5), Kim Oanh VT(1), Minh Hang NT(1), Van HN(1), Le TN(1).
Author information:
(1)Institute of Marine Biochemistry and Graduate University of Science and Technology, Vietnam Academy of Science and Technology
(VAST), Caugiay, Vietnam.
(2)School of Medicine and Pharmacy, Vietnam National University, Caugiay, Vietnam.
(3)Traditional Medicine Hospital of Public Security Ministry of Vietnam, Hanoi, Vietnam.
(4)Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Sofia, Bulgaria.
(5)Thuyloi University, Dongda, Vietnam.


The n-hexane extract of Knema pachycarpa fruits (Myristicaceae family), exhibiting strong anti-acetylcholinesterase activity, was investigated by gas chromatography/mass spectrometry and then purified by column chromatography. Guided by GC/MS profiling and bioassay, chromatographic separations led to the isolation of five new compounds: two anacardic acid derivatives 1-2, two cardanol derivatives 3-4 and a cardol derivative 5, along with mixtures of known phenolic lipids 6-9. The chemical structures were determined by various spectroscopic methods. New isolated compounds 1-5 were evaluated for their cytotoxicity and anti-acetylcholinesterase activity. Cardanol 3 and cardol 5 were the most active compounds in the acetylcholinesterase inhibitory assay with IC50 values of 2.60 ± 0.24 µM and 2.46 ± 0.23 µM, respectively. Cardanol 4 and cardol 5 showed moderate cytotoxicity against Hela and MCF-7 cancer cell lines with IC50 values ranging from 31.36 ± 0.41 µM to 41.30 ± 2.49 µM.