Cloning of PCS gene (TpPCS1) from Tagetes patula L. and expression analysis under cadmium stress.

Affiliation

Zha YQ(1), Zhang KK(2), Pan F(1), Liu X(1), Han SM(1), Guan P(1).
Author information:
(1)Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region
(Ministry of Education), Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering
(CICMEAB), College of Life Sciences/Institute of Agro-bioengineering, Guizhou University, Guiyang, China.
(2)Guizhou Animal Husbandry and Veterinary Research Institute, Guizhou Provincial Academy of Agricultural Sciences, Guiyang, China.

Abstract

Phytochelatins (PCs) constitute an important mechanism for plants to resist heavy metal stress. Widely found in higher plants, they are small heavy metal binding peptides, synthesized through catalysis of phytochelatin synthase (PCS). We speculate that there may be PCS genes in Peacock grass (Tagetes patula L., Asteraceae), which is an important reason for its rich cadmium. In order to obtain the full-length cDNA sequence of the PCS gene from T. patula L. used rapid amplification of cDNA ends (RACE). Meanwhile, Relative expression of TpPCS1 under different concentrations of cadmium (Cd) stress was analysed using quantitative real-time polymerase chain reaction (qRT-PCR). Results found ORF of TpPCS1 genes with a length of 1970 bp, a gene coding area length of 1764 bp, coding for 587 amino acids. Expression of TpPCS1 under Cd stress was tissue specific. TpPCS1 in the root showed higher expression, while expression in the leaf and seed was relatively low. This research demonstrates that expression of TpPCS1 enhanced the enrichment of cadmium in T. patula L. roots and could be used to construct a plant hyperexpression carrier that would provide new avenues for plant restoration technology.