The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China; Research Centre of Industrial Microbiology, School of Biotechnology, Jiangnan University, Wuxi 214122, People's Republic of China. Electronic address: [Email]
The aim of this work was to realize 1,2,4-butantriol (BT) production from sugarcane bagasse hydrolysates by microbial fermentation, and obtain co-production of BT and ethanol. Candida glycerinogenes UG21 was utilized to reduce the effect of osmolality resulting from high glucose concentration and furfural in hydrolysates on cell growth of BT-producing strains, and produced 54.1 g/L ethanol from glucose. After ethanol recovering, xylose containing stillage was obtained and used for BT production. 1.3 g/L BT was generated by a BT-producing strain. By the deletion of the crr gene and process optimization, BT titer reached 4.9 g/L. Meanwhile, the efficient utilization of sugarcane bagasse was achieved by a two-stage fermentation for co-production of BT and ethanol. This study provided a novel strategy for BT production from sugarcane bagasse, and demonstrated the potential for making full use of sugarcane bagasse hydrolysates to co-production value-added products.