Complete nucleotide sequence of chrysanthemum mosaic-associated virus, a novel emaravirus infecting chrysanthemum.

Affiliation

Kubota K(1), Yanagisawa H(2)(3), Chiaki Y(2)(4), Yamasaki J(5), Horikawa H(6), Tsunekawa K(6), Morita Y(5), Kadono F(7).
Author information:
(1)Central Region Agricultural Research Center, NARO, Kannondai, Tsukuba, Ibaraki, 305-8666, Japan. [Email]
(2)Central Region Agricultural Research Center, NARO, Kannondai, Tsukuba, Ibaraki, 305-8666, Japan.
(3)Narita Branch, Yokohama Plant Protection Station, Aza-Tennamino, Komaino, Narita, Chiba, 282-0021, Japan.
(4)Grape and Persimmon Research Station, Institute of Fruit tree and Tea Science, NARO, Higashi-Hiroshima, Hiroshima, Akitsu, 739-2494, Japan.
(5)Kochi Agricultural Research Center, Hataeda, Nankoku, Kochi, 783-0023, Japan.
(6)Aichi Agricultural Research Center, Sagamine, Yazako, Nagakute, Aichi, 480-1193, Japan.
(7)Department of Clinical Plant Science, Faculty of Bioscience and Applied Chemistry, Hosei University, Kajino-cho, Koganei, Tokyo, 184-8584, Japan.

Abstract

Here, we report the complete genome sequence of chrysanthemum mosaic-associated virus (ChMaV), a putative new member of the genus Emaravirus. The ChMaV genome comprises seven negative-sense RNA segments (RNAs 1, 2, 3a, 3b, 4, 5, and 6), and the amino acid sequences of its RNA-dependent RNA polymerase (RNA1), glycoprotein precursor (RNA2), nucleocapsid protein (RNA3), and movement protein (RNA4) showed the closest relationship to pear chlorotic leaf spot-associated virus. Phylogenetic analysis showed that it clusters with emaraviruses whose host plants originate from East Asia.