Crateriforma spongiae sp. nov., isolated from a marine sponge and emended description of the genus "Crateriforma".

Affiliation

Kumar G(1), Kumar D(1), Jagadeeshwari U(2), Sreya PK(1), Shabbir A(1), Sasikala C(3), Ramana CV(4).
Author information:
(1)Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad, 500046, India.
(2)Bacterial Discovery Laboratory,Centre for Environment, Institute of Science and Technology, JNT University Hyderabad, Kukatpally, Hyderabad, 500085, India.
(3)Bacterial Discovery Laboratory,Centre for Environment, Institute of Science and Technology, JNT University Hyderabad, Kukatpally, Hyderabad, 500085, India. [Email]
(4)Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad, 500046, India. [Email]

Abstract

A Gram-stain-negative, aerobic, pear to oval shaped, rosette forming bacterium with crateriform structures well distributed on the cell surface designated as strain JC647T was isolated from a sponge specimen belonging to the genus Spongia. Strain JC647T reproduces through budding. Strain JC647T shared highest 16S rRNA gene sequence identity of 99.9% with "Crateriforma conspicua" Mal65T (not a valid species name). The genome size of strain JC647T is 6.9 Mb with a G + C content of 57.8 mol %. For the resolution of the phylogenetic congruence of the novel strain, the phylogeny was also constructed with the sequences of ninety-two housekeeping genes. Based on the phylogenetic analyses, low dDDH value (51.0%), low gANI (93.2%), low AAI (94.9%) results, chemotaxonomic characteristics and differential physiological properties, strain JC647T is recognized as a new species of the genus "Crateriforma", for which we propose the name Crateriforma spongiae sp. nov. The type species is JC647T (= KCTC 72176T = NBRC 114068T).