Development of a loop-mediated isothermal amplification assay for Vibrio cholerae O1 and O139.

Affiliation

Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan. Electronic address: [Email]

Abstract

A loop-mediated isothermal amplification assay was developed. It was designed for recognizing Vibrio cholerae O1/O139, where atpA, rfbN, and wfbR genes were adopted. The assay specifically detected the target with sensitivities of 5-67 copies per reaction in 1 h. The assay will aid rapid detection of the cholera bacterium.

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