Development of a methodology for reversible chemical modification of silicon surfaces with application in nanomechanical biosensors.


Centro de Investigaciones y Transferencia Villa María (CIT VM - CONICET), Instituto de Ciencias Básicas y Aplicadas, Universidad Nacional de Villa María, Av. Arturo Jauretche 1555, Villa María, C.P, 5900, Córdoba, Argentina. Electronic address: [Email]


Hypervalent tellurium compounds have a particular reactivity towards thiol compounds which are related to their biological properties. In this work, this property was assembled to tellurium-functionalized surfaces. These compounds were used as linkers in the immobilization process of thiolated biomolecules (such as DNA) on microcantilever surfaces. The telluride derivatives acted as reversible binding agents due to their redox properties, providing the regeneration of microcantilever surfaces and allowing their reuse for further biomolecules immobilizations, recycling the functional surface. Initially, we started from the synthesis of 4-((3-((4-methoxyphenyl) tellanyl) phenyl) amino)-4-oxobutanoic acid, a new compound, which was immobilized on a silicon surface. In nanomechanical systems, the detection involved a hybridization study of thiolated DNA sequences. Fluorescence microscopy technique was used to confirm the immobilization and removal of the telluride-DNA system and provided revealing results about the potentiality of applying redox properties to chalcogen derivatives at surfaces.


Biosensor,Chalcogen chemistry,Microcantilever,Nanomechanical biosensors,Reversible immobilization,Surface regeneration,

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