Development of sequential and simultaneous bacterial cultures to hydrolyse and detoxify wood pre-hydrolysate for enhanced acetone-butanol-ethanol (ABE) production.


Department of Chemistry and Chemical Engineering, Royal Military College of Canada, 13 General Crerar Crescent, Kingston, ON, K7K 7B4, Canada. Electronic address: [Email]


The use of microorganisms is a promising option for an eco-efficient and successful conversion of hardwood hemicelluloses to biofuels. The focus of this work is the treatment of hemicellulosic pre-hydrolysate by flocculation, followed by simultaneous or separate detoxification with Ureibacillus thermosphaericus and Cupriavidus taiwanensis co-culture, and hydrolysis with Paenibacillus campinasensis. A reduction of phenolic compounds was achieved mainly after flocculation, applied as a first detoxification step, but no increase in sugars concentration was observed. The ABE fermentation of the hydrolysate obtained from the simultaneous hydrolysis and detoxification produced 6.8 g L-1 of butanol after 116 h, which was higher than that generated with xylose synthetic medium. The higher biofuel concentration in the hydrolysate is attributed to the existence of carbon sources, other than xylose.


ABE fermentation,Hemicelluloses,Hydrolysis,Microbial consortium,Phenolic compounds,

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