Digital image colorimetric analysis for evaluating lipid oxidation in oils and its emulsion.


Department of Chemistry, Faculty of Science and Technology, Thammasat University, 99 Moo 18 Paholyothin Road, Klong Luang, Pathumthani 12121, Thailand. Electronic address: [Email]


Optical analytical images were captured by a digital camera, and lipid peroxidation was monitored using colorimetric determination. The Image-J method was shown to have comparable accuracy to the conventional titration (American Oil Chemists' Society) and UV-Vis spectroscopic methods. Over the PV range 3-14 meq.O2/kg, a good correlation (R2 = 0.9836) was found between the proposed method and UV-Vis method with an SD of ±0.0339 meq.O2/kg. The range and accuracy of detection were determined from the changes in the Image-J intensity values. At equivalent PV, the limit of detection was 0.175 meq.O2/kg and the limit of quantitation was 0.35 meq.O2/kg. The proposed Image-J application can be used to develop fast and portable devices for monitoring peroxide values. These have potential applications in quality control for small-scale farmers and small and medium enterprises.


Colorimetric determination,Lipid peroxidation,Optical analytical image,Peroxide value,

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