Effect of lipid chain length on nanostructured lipid carriers: Comprehensive structural evaluation by scattering techniques.

Affiliation

Stranski Laboratorium für Physikalische und Theoretische Chemie, Institut für Chemie, Technische Universität Berlin, D-10623 Berlin, Germany. Electronic address: [Email]

Abstract

The objective of the present investigation was to evaluate nano-dispersed systems of differently chained lipids (solid phases) using various scattering techniques. Nanostructured lipid carriers (NLCs) were fabricated by employing the microemulsification methodology in which dialkyldimethyl ammonium bromide (DxDAB) of different alkyl chain length (x = 12, 14, 16, 18) and oleic acid were used as the solid lipid and liquid lipid, respectively. For the first time, the effect of DxDAB as a function of the chain length of the double alkyl chain on the structural characteristics of NLCs was investigated. Dynamic light scattering (DLS) and static light scattering (SLS) results showed that a small but systematic size increase occurs with increase in the chain length 'x' of the DxDAB from 12 to 16, yet D18DAB based NLCs exhibited the smallest size irrespective of its higher hydrophobicity. Small angle neutron scattering (SANS) analysis revealed the structural make up of NLCs having spherical nanoparticles and triaxial ellipsoidal core-shell micelles in the system. In-vitro cytotoxicity evaluation indicated that toxicity is simply concentration-dependent phenomena and NLCs with less than 5 mg/mL are preferred for better in-vivo tolerance.

Keywords

D(x)DAB nanoparticles,Dynamic light scattering,Hemolysis,Molecular weight of aggregates,Nanostructured lipid carriers,Small-angle neutron scattering,Static light scattering,