Efficacy of low pressure DBD plasma in the reduction of T-2 and HT-2 toxin in oat flour.

Affiliation

Croatian Veterinary Institute, Veterinary Institute Križevci, Zakmardijeva 10, 48260 Križevci, Croatia. Electronic address: [Email]

Abstract

T-2 and its major metabolite HT-2 toxin are naturally occurring contaminants in cereals, with the highest concentrations determined in oats. Because of their toxicity and resistance to conventional methods used in mycotoxin degradation, development of new effective procedures for reduction of T-2/HT-2 toxin levels is needed. The aim of this study was to investigate the effect of different gas types within various timeframes on the efficiency of T-2 and HT-2 toxin degradation by low pressure dielectric barrier discharge (DBD) plasma in oat flour. Although humidity of the sample influences the intensity of the plasma, oxygen atoms efficiently oxidize the sample and produce CO and N2. Before and after treatment, T-2 and HT-2 toxin concentrations were analysed by the confirmatory liquid chromatography tandem-mass spectrometry (LC-MS/MS) method. The highest reduction of T-2 and HT-2 toxin of 43.25% and 38.54%, respectively, was achieved using nitrogen plasma and this proved to be time-dependent.

Keywords

Cold plasma,HT-2 toxin,HT-2 toxin (PubChem CID: 10093830),Mycotoxin reduction,Oats,T-2 toxin,T-2 toxin (PubChem CID: 5284461),

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