Efficient biosynthesis of (S)-1-[2-(trifluoromethyl)phenyl]ethanol by a novel
isolate Geotrichum silvicola ZJPH1811 in deep eutectic
solvent/cyclodextrin-containing system.
Xiong L(1), Kong X(1), Liu H(1), Wang P(2). Author information:
(1)Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of
Ministry of Education, College of Pharmaceutical Science, Zhejiang University of
Technology, Hangzhou 310014, People's Republic of China.
(2)Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of
Ministry of Education, College of Pharmaceutical Science, Zhejiang University of
Technology, Hangzhou 310014, People's Republic of China. Electronic address:
[Email]
This study aimed to develop a biotransformation process for the production of (S)-1-[2-(trifluoromethyl)phenyl]ethanol, a key chiral intermediate of Plk1 inhibitor, and increase its productivity through medium engineering strategy. A fungus isolate Geotrichum silvicola ZJPH1811 was adopted as biocatalyst for 2'-(trifluoromethyl)acetophenone reduction, and gave the best performance with > 99.2% product ee. To improve the yield, choline acetate/cysteine (ChAc/Cys) was introduced as co-solvent in reaction system, which accelerated mass transfer and protected cells from substrate inhibition. Moreover, a synergistic effect of methylated-β-cyclodextrin (MCD) and ChAc/Cys was found in the bioreduction, with further enhancement in substrate concentration and cell membrane permeability. Compared with buffer system, in the developed ChAc/Cys-MCD-containing system, substrate loading and product yield were increased by 6.7-fold and 2.4-fold respectively. This is the first report on (S)-1-[2-(trifluoromethyl)phenyl]ethanol production with G. silvicola, and provides valuable insight into the synergistic effect of DES and CDs in biocatalysis.
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