Electron injection from mitochondrial transcription factor A to DNA associated with thymine dimer photo repair.


Department of Chemistry, Graduate School of Science, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo, Kyoto 606-8502, Japan; Institute for Integrated Cell-Material Sciences, Institute for Advanced Study, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan. Electronic address: [Email]


Electron transfer through π-stacked arrays of double-stranded DNA contributes to the redox chemistry of bases, including guanine oxidation and thymine-thymine dimer repair by photolyase. 5-Bromouracil is an attractive photoreactive thymine analogue that can be used to investigate electron transfer in DNA, and is a useful probe for protein-DNA interaction analysis. In the present study using BrU we found that UV irradiation facilitated electron injection from mitochondrial transcription factor A into DNA. We also observed that this electron injection could lead to repair of a thymine-thymine dimer.


Bromouracil,Electron transfer,Mitochondria,Photochemical,TFAM,