Engineering bioorthogonal protein-polymer hybrid hydrogel as a functional protein immobilization platform.


Department of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA. [Email]


We demonstrate the synthesis of protein-polymer hybrid hydrogel that can be used as a platform for immobilizing functional proteins. Orthogonal chemistry was employed for cross-linking the hybrid network and conjugating proteins to the gel backbone, allowing for the convenient, one-pot formation of a functionalized hydrogel. The resulting hydrogel had tunable mechanical properties, was stable in solution, and biocompatible.