Evaluation of UV-C Decontamination of Clinical Tissue Sections for Spatially Resolved Analysis by Mass Spectrometry Imaging (MSI).


Dannhorn A(1)(2), Ling S(2), Powell S(3), McCall E(3), Maglennon G(4), Jones GN(5), Pierce AJ(5), Strittmatter N(2), Hamm G(2), Barry ST(6), Bunch J(7), Goodwin RJA(2)(8), Takats Z(1).
Author information:
(1)Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, 605 SAF Building, South Kensington Campus, London CB4 0FZ, U.K.
(2)Imaging and Data Analytics, Clinical Pharmacology and Safety Sciences, R&D, AstraZeneca, Cambridge SW7 2AZ, U.K.
(3)Safety, Health and Environment
(SHE), Cambridge Operations, BioPharmaceuticals R&D, AstraZeneca, Cambridge CB4 0FZ, U.K.
(4)Oncology Safety, Clinical Pharmacology and Safety Sciences, R&D, AstraZeneca, Cambridge CB22 3AT, U.K.
(5)Translational Medicine, Oncology R&D, AstraZeneca, Cambridge SG8 6EH, U.K.
(6)Bioscience, Discovery, Oncology R&D, AstraZeneca, Cambridge CB2 0RE, U.K.
(7)National Centre of Excellence in Mass Spectrometry Imaging
(NiCE-MSI), National Physical Laboratory, Teddington TW11 0LW, U.K.
(8)Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8QQ, U.K.


Clinical tissue specimens are often unscreened, and preparation of tissue sections for analysis by mass spectrometry imaging (MSI) can cause aerosolization of particles potentially carrying an infectious load. We here present a decontamination approach based on ultraviolet-C (UV-C) light to inactivate clinically relevant pathogens such as herpesviridae, papovaviridae human immunodeficiency virus, or SARS-CoV-2, which may be present in human tissue samples while preserving the biodistributions of analytes within the tissue. High doses of UV-C required for high-level disinfection were found to cause oxidation and photodegradation of endogenous species. Lower UV-C doses maintaining inactivation of clinically relevant pathogens to a level of increased operator safety were found to be less destructive to the tissue metabolome and xenobiotics. These doses caused less alterations of the tissue metabolome and allowed elucidation of the biodistribution of the endogenous metabolites. Additionally, we were able to determine the spatially integrated abundances of the ATR inhibitor ceralasertib from decontaminated human biopsies using desorption electrospray ionization-MSI (DESI-MSI).