The current work describes the influence of sintering temperatures on biological and mechanical properties of naturally derived hydroxyapatite (HAp). The phase pure hydroxyapatite developed from the goat bone has been obtained by optimizing the calcination temperature from 600-900 °C. Further, HAp calcined at 900 °C was subjected to various sintering temperature (1100-1400 °C). Finally, the influence of sinter temperatures on mechanical (hardness) and biological properties (in vitro bioactivity, MTT and hemocompatibility assays) were ascertained. In respect of biological properties, it came to know that 1300 °C is optimum sinter temperature, which has enhanced apatite growth with the superior cell viability and hemo-compatible behavior. However, sample sintered at 1400 °C delivers maximum hardness. Thus, the hydroxyapatite extracted from goat bone can find better applications in bio-medical engineering as analogous to the existing man-made synthetic materials.