Formulation of a pH-sensitive cancer cell-targeted gene delivery system based on folate-chitosan conjugated nanoparticles.

Affiliation

Mohammadzadeh R(1), Shahim P(1), Akbari A(2).
Author information:
(1)Department of Cell and Molecular Biology, Faculty of Science, University of Maragheh, Maragheh, Iran.
(2)Solid Tumor Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran.

Abstract

In this study, we investigated the design and construct of a chitosan (CA)-based targeted gene delivery system and evaluated its function. To this end, CA-folic acid/pDNA (CA-FA/pDNA) nanoparticles were prepared in different formulations using the ion gelation method. All the synthesized nanoparticles were characterized using FTIR, TEM, SEM and DLS. Moreover, the effects of molecular weight (MW) of CA, DNA, and CA concentration were inspected on encapsulation efficiency (EE). The results showed that the EE of pDNA was directly proportional with MW of CA and CA concentration but was in an inverse proportion with DNA concentration. In addition, high MW of CA and low MW of CA nanoparticles showed lower and higher pDNA release in all pH ranges, respectively. It is concluded that the N/P ratio increase can cause controlled pDNA release.