Galectin-1 Inhibited LPS-Induced Autophagy and Apoptosis of Human Periodontal Ligament Stem Cells.

Affiliation

Zhang J(1), Dong X(2), Yan Q(3), Ren W(1), Zhang R(1), Jiang X(4), Geng Z(1), Xu X(1), Liu C(1), Zhang S(5), Liu D(6), Liu Y(7)(8).
Author information:
(1)Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, No. 44-1 West Wenhua Road, Jinan, Shandong, 250012, People's Republic of China.
(2)Changle People's Hospital, Weifang, Shandong, 262400, People's Republic of China.
(3)Qufu Stomatological Hospital, Jining, Shandong, 273100, People's Republic of China.
(4)Department of Pharmaceutics, Key Laboratory of Chemical Biology
(Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong, 250012, People's Republic of China.
(5)Department of Orthodontics, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, People's Republic of China.
(6)Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, No. 44-1 West Wenhua Road, Jinan, Shandong, 250012, People's Republic of China. [Email]
(7)Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, No. 44-1 West Wenhua Road, Jinan, Shandong, 250012, People's Republic of China. [Email]
(8)Shenzhen Research Institute of Shandong University, Shenzhen, Guangdong, 518057, People's Republic of China. [Email]

Abstract

Periodontitis is a widespread human chronic inflammatory disease of the tooth-surrounding tissues, which induces the destruction of periodontium and pathologic loss of teeth among adults. It has been reported that interleukin (IL)-17 was significantly increased in periodontitis patients compared to controls, while galectin-1 (Gal-1) was lower. Interestingly, it is found that Gal-1 treatment reduced systemic IL-17 levels. Hence, the aim of the present study was to explore the effect of Gal-1 on periodontitis development and investigate its underlying mechanism. In this study, Gal-1 was poorly expressed in lipopolysaccharide (LPS)-induced human periodontal ligament stem cells (hPDLSCs), and Gal-1 overexpression attenuated the production of inflammatory cytokines induced by LPS. Moreover, Gal-1 overexpression alleviated LPS-induced cell autophagy and apoptosis and reduced the expressions of IL-17A and IL-17R. Interestingly, IL-17A reversed the effect of Gal-1 on cell autophagy, inflammation, and cell apoptosis induced by the LPS challenge. In conclusion, Gal-1 inhibited LPS-induced autophagy and apoptosis of hPDLSC via regulation of IL-17A expression. Therefore, Gal-1 may have promising potential in regenerating periodontium.