Genotoxic and Cytotoxic Properties of Zinc Oxide Nanoparticles Phyto-Fabricated from the Obscure Morning Glory Plant Ipomoea obscura (L.) Ker Gawl.

Affiliation

Murali M(1), Anandan S(2)(3), Ansari MA(4), Alzohairy MA(5), Alomary MN(6), Asiri SMM(7), Almatroudi A(5), Thriveni MC(8), Singh SB(9), Gowtham HG(9), Aiyaz M(8), Srinivasa C(10), Urooj A(2), Amruthesh KN(1).
Author information:
(1)Applied Plant Pathology Laboratory, Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India.
(2)Department of Studies in Food Science and Nutrition, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India.
(3)Department of Clinical Nutrition and Dietetics, Sri Devaraj Urs Academy of Higher Education and Research, Kolar 563101, Karnataka, India.
(4)Department of Epidemic Disease Research, Institutes for Research and Medical Consultations
(IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia.
(5)Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Qassim 51431, Saudi Arabia.
(6)National Center for Biotechnology, Life Science and Environmental Research Institute, King Abdulaziz City for Science and Technology, Riyadh P.O. Box 6086, Saudi Arabia.
(7)Department of Biophysics, Institute for Research and Medical Consultations
(IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia.
(8)Central Sericultural Germplasm Resources Centre, Central Silk Board, Ministry of Textiles, Thally Road, TVS Nagar, Hosur 635109, Tamil Nadu, India.
(9)Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India.
(10)Department of Studies in Biotechnology, Davangere University, Davangere 577007, Karnataka, India.

Abstract

The study was undertaken to investigate the antioxidant, genotoxic, and cytotoxic potentialities of phyto-fabricated zinc oxide nanoparticles (ZnO-NPs) from Ipomoea obscura (L.) Ker Gawl. aqueous leaf extract. The UV-visible spectral analysis of the ZnO-NPs showed an absorption peak at 304 nm with a bandgap energy of 3.54 eV, which are characteristics of zinc nanoparticles. Moreover, the particles were of nano-size (~24.26 nm) with 88.11% purity and were agglomerated as observed through Scanning Electron Microscopy (SEM). The phyto-fabricated ZnO-NPs offered radical scavenging activity (RSA) in a dose-dependent manner with an IC50 of 0.45 mg mL-1. In addition, the genotoxicity studies of ZnO-NPs carried out on onion root tips revealed that the particles were able to significantly inhibit the cell division at the mitotic stage with a mitotic index of 39.49%. Further, the cytotoxic studies on HT-29 cells showed that the phyto-fabricated ZnO-NPs could arrest the cell division as early as in the G0/G1 phase (with 92.14%) with 73.14% cells showing early apoptotic symptoms after 24 h of incubation. The results of the study affirm the ability of phyto-fabricated ZnO-NPs from aqueous leaf extract of I. obscura is beneficial in the cytotoxic application.