Histological study and immunohistochemical location of cytoskeletal proteins in the ovaries of the three species of lizards of the family Leiosauridae (Reptilia: Squamata).

Affiliation

da Silveira Firmiano EM(1), Machado-Santos C(2), Serra-Campos AO(3), Maria de Sousa B(4), Pinheiro NL(5), do Nascimento AA(6).
Author information:
(1)Universidade Federal Rural do Rio de Janeiro, Research Postgraduate Program in Animal Biology Institute of Biological and Health Sciences, BR 465, Km 7, Seropédica, RJ CEP 23890-000, Brazil. Electronic address: [Email]
(2)Laboratory of Teaching and Research in Histology and Compared Embryology
(LEPHEC), Federal Fluminense University, Niterói, RJ, CEP 24210-130, Brazil. Electronic address: [Email]
(3)Laboratory of Teaching and Research in Histology and Compared Embryology
(LEPHEC), Federal Fluminense University, Niterói, RJ, CEP 24210-130, Brazil. Electronic address: [Email]
(4)Herpetology Laboratory, Department of Zoology, Institute of Biological Sciences, Federal University of Juiz de Fora-UFJF, University Campus No Number, Martelos, Juiz de Fora, MG, CEP 36036-900, Brazil. Electronic address: [Email]
(5)Universidade Federal Rural do Rio de Janeiro, Research Postgraduate Program in Animal Biology Institute of Biological and Health Sciences, BR 465, Km 7, Seropédica, RJ CEP 23890-000, Brazil. Electronic address: [Email]
(6)Universidade Federal Rural do Rio de Janeiro, Research Postgraduate Program in Animal Biology Institute of Biological and Health Sciences, BR 465, Km 7, Seropédica, RJ CEP 23890-000, Brazil. Electronic address: [Email]

Abstract

The aim of the study is to expand information on the histological aspect, presence, and distribution of cytoskeletal proteins: smooth muscle alpha-actin (α-SMA), desmin and vimentin in ovaries of three lizards Leiosauridae. In all analysed species, the ovaries were paired organs, located inside the coelomic cavity, covered in a simple cubic epithelium. Below the surface was the tunica albuginea. The organs can be divided into two regions: the cortex and the medulla. The pre-vitellogenic follicles consist of an oocyte surrounded by the pellucid zone periodic acid schiff positive, the granulosa layer consisting of three cell types: small, intermediate, and large and the theca layer. The vitellogenic follicles presented only a single layer of cubic granulosa cells. In the three lizards, α-SMA microfilaments (MFs) were verified along the theca layer and in endothelial cells of the blood vessels of the analysed follicles. Researchers have observed desmin intermediate filaments immunostaining exclusively muscle fibers of the albuginea and the ovarian stroma of Enyalius perditus. There was no immunostaining for vimentin in the ovaries of all the lizards studied. Results obtained revealed that the MFs of α-SMA could be responsible for a contractile activity present in the lizards assessed.