Immortalization of Human Keratinocytes Using the Catalytic Subunit of Telomerase.

Affiliation

Beilin AK(1)(2), Gurskaya NG(1)(2), Evtushenko NA(2), Alpeeva EV(1), Kosykh AV(1)(2), Terskikh VV(1), Vasiliev AV(1)(3), Vorotelyak EA(4)(5)(6).
Author information:
(1)Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia.
(2)Pirogov Russian National Research Medical University, Moscow, Russia.
(3)Moscow State University, Moscow, Russia.
(4)Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, Russia. [Email]
(5)Pirogov Russian National Research Medical University, Moscow, Russia. [Email]
(6)Moscow State University, Moscow, Russia. [Email]

Abstract

A new stable line of human keratinocytes was obtained. The cells have altered morphology, both abnormal chromosomal composition and expression of keratinocyte markers, do not show contact inhibition, could be cultured in various media and have limited stratification ability in vitro. Upon transplantation into nude mice the cells have tumorigenic properties.