Impact of Glycans on Lipid Membrane Dynamics at the Nanoscale Unveiled by Planar Plasmonic Nanogap Antennas and Atomic Force Spectroscopy.

Affiliation

Winkler PM(1), Campelo F(1), Giannotti MI(2)(3)(4), Garcia-Parajo MF(1)(5).
Author information:
(1)ICFO-Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, 08860 Barcelona, Spain.
(2)Biomedical Research Networking Center on Bioengineering, Biomaterials and Nanomedicine
(CIBER-BBN), 28029 Madrid, Spain.
(3)Institut de Bioenginyeria de Catalunya
(IBEC), The Barcelona Institute of Science and Technology, 08860 Barcelona, Spain.
(4)Universitat de Barcelona
(UB), 08007 Barcelona, Spain.
(5)ICREA, Pg. Lluís Companys 23, 08010 Barcelona, Spain.

Abstract

Lateral compartmentalization of the plasma membrane is a prominent feature present at multiple spatiotemporal scales that regulates key cellular functions. The extracellular glycocalyx matrix has recently emerged as an important player that modulates the organization of specific receptors and patterns the lipid bilayer itself. However, experimental limitations in investigating its impact on the membrane nanoscale dynamics have hampered detailed studies. Here, we used photonic nanoantenna arrays combined with fluorescence correlation spectroscopy to investigate the influence of hyaluronic acid (HA), a prominent glycosaminoglycan, on the nanoscale organization of mimetic lipid bilayers. Using atomic force microscopy and force spectroscopy, we further correlated our dynamic measurements with the morphology and mechanical properties of bilayers at the nanoscale. Overall, we find that HA has a profound effect on the dynamics, nanoscale organization, and mechanical properties of lipid bilayers that are enriched in sphingolipids and/or cholesterol, such as those present in living cells.