Improved CRISPR/Cas9 gene editing by fluorescence activated cell sorting of green fluorescence protein tagged protoplasts.


Department of Plant and Environmental Sciences, University of Copenhagen, DK-1871, Frederiksberg C, Denmark. [Email]


CRISPR/Cas9 is widely used for precise genetic editing in various organisms. CRISPR/Cas9 editing may in many plants be hampered by the presence of complex and high ploidy genomes and inefficient or poorly controlled delivery of the CRISPR/Cas9 components to gamete cells or cells with regenerative potential. Optimized strategies and methods to overcome these challenges are therefore in demand.


CRISPR/Cas9,Fluorescence activated cell sorting,Genome engineering,Mutation enrichment,Nicotiana benthamiana,Precise genetic editing,Protoplasting,