Improved method for tuberculocidal and mycobactericidal activity testing of disinfectants based on the European Standard EN 14348.


Brill FHH(1), Lenz J(2), Lach C(2), Radischat N(3), Paßvogel L(3), Goroncy-Bermes P(3), Gabriel H(1), Steinmann J(4), Steinhauer K(5).
Author information:
(1)Dr. Brill + Partner GmbH Institut für Hygiene und Mikrobiologie, Hamburg, Germany.
(2)Chemische Fabrik Dr. Weigert GmbH & Co. KG, Hamburg, Germany.
(3)Department Research & Scientific Services, Schülke & Mayr GmbH, Norderstedt, Germany.
(4)Institute of Clinical Hygiene, Medical Microbiology and Infectiology, General Hospital Nürnberg, Paracelsus Medical University, Nürnberg, Germany.
(5)Department Research & Scientific Services, Schülke & Mayr GmbH, Norderstedt, Germany; Faculty of Mechanical Engineering, Kiel University of Applied Sciences, Kiel, Germany. Electronic address: [Email]


Safe measurements to prevent the transmission of (multidrug-resistant) mycobacteria such as disinfection are essential in healthcare settings. In Europe chemical disinfectants are tested for their tuberculocidal and mycobactericidal efficacy by the internationally accepted test procedure described in EN 14348. However, especially for amine-based disinfectants, invalid results may occur by this procedure due to insufficient neutralization. In this multi-laboratory study the procedure described in EN 14348 was optimized by a combination of chemical neutralization and membrane filtration in order to obtain a valid and secure method especially for amine-based disinfectants.