Njeru SN(1)(2), Muema JM(3). Author information:
(1)Department of Biochemistry, School of Health Sciences, Kisii University, P.O.
Box 408-40200, Kisii, Kenya. [Email]
(2)Division of Immunology, Paul-Ehrlich-Institute, Federal Institute for
Vaccines and Biomedicines, Paul-Ehrlich-Straße 51-59, 63225, Langen, Hessen,
(3)Department of Biochemistry, Jomo Kenyatta University of Agriculture and
Technology (JKUAT), P.O. Box 62000-00200, Nairobi, Kenya.
OBJECTIVES: We and others have shown that Aspilia pluriseta is associated with various biological activities. However, there is a lack of information on its cytotoxicity. This has created an information gap about the safety of A. pluriseta extracts. As an extension to our recent publication on the antimicrobial activity and the phytochemical characterization of A. pluriseta root extracts, here we report on cytotoxicity of tested solvent fractions. We evaluated the potential cytotoxicity of these root extract fractions on Vero cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. RESULTS: We show that all solvent extract fractions (except methanolic solvent fractions) had cytotoxic concentration values that killed 50% of the Vero cells (CC50) greater than 20 µg/mL and selectivity index (SI) greater than 1.0. Taken together, we demonstrate that, A. pluriseta extract fractions' earlier reported bioactivities are within the acceptable cytotoxicity and selective index limits. This finding scientifically validates the potential use of A. pluriseta in the discovery of safe therapeutics agents.
Having over 250 Research scholars worldwide and more than 400 articles online with open access.