Peng JY(1), Botelho MG(2), Matinlinna JP(3), Pan HB(4), Kukk E(5), Low KJ(6). Author information:
(1)Nanjing Stomatological Hospital, Medical School of Nanjing University,
Nanjing, P.R. China.
(2)Oral Rehabilitation, Faculty of Dentistry, The University of Hong Kong, Hong
Kong Special Administrative Region, P.R. China.
(3)Dental Materials Science, Faculty of Dentistry, The University of Hong Kong,
Hong Kong Special Administrative Region, P.R. China.
(4)Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced
Technology, Chinese Academy of Sciences, Shenzhen, P.R. China.
(5)Department of Physics and Astronomy, Faculty of Mathematics and Natural
Sciences, University of Turku, Turku, Finland.
(6)HKU-CAS Joint Laboratory on New Materials, State Key Laboratory of Synthetic
Chemistry and Department of Chemistry, the University of Hong Kong, Hong Kong
Special Administrative Region, P.R. China.
OBJECTIVE: The effects of saliva on demineralized dentin and silver diamine fluoride (SDF) were investigated in vitro. METHODS: Dentin samples stored in deionized water (DIW), buffer solution (BS), basal medium mucin (BMM), and unstimulated whole saliva (UWS) were demineralized for 3 days and immersed in the same storage media. SDF as a 38 mass% solution was applied to the dentin samples for 3 minutes after they had been replaced in their respective medium. Surfaces were analyzed by scanning electron microscopy, energy-dispersive X-ray analysis (EDX), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD). RESULTS: Scanning electron microscopy showed various surface deposits and coatings, including occlusion of dentinal tubules. DIW resulted in the thinnest coating, whereas BMM resulted in the thickest. EDX and XPS showed the formation of metallic silver and silver compounds in all four media, with the greatest formation in BS. XRD indicated that the main product was silver chloride except in DIW. Sulphur was found in BMM and UWS. EDX and XPS detected fluoride and XRD detected calcium fluoride and fluorohydroxyapatite in BS, BMM, and UWS. CONCLUSION: The interaction between SDF and demineralized dentin was dependent upon the storage medium. BMM provided an outcome most similar to human saliva.
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