Investigation of BRAF mutation in a series of papillary thyroid carcinoma and matched-lymph node metastasis with ARMS PCR.

Affiliation

Department of Surgery, Binzhou People's Hospital, No. 515 Huanghe 7th Road, Binzhou, Shandong Province, 256600, PR China. Electronic address: [Email]

Abstract

OBJECTIVE : To figure out that if there is a consistency relationship of the BRAFV600E mutation in matched-lymph node metastasis and original papillary thyroid carcinoma (PTC) specimen for the same patient.
METHODS : We collected the specimen of thyroids and matched-lymph node metastases of PTCs and tested the BRAFV600E mutation status with amplification refractory mutation system (ARMS) PCR.
RESULTS : 20 patients with PTC and metastasis lymph node were hired. In this cohort, 16 (80%) patients had the same BRAF genetic mutation status in thyroid and metastasis, and the other 4 (20%) had an inconsistent situation.
CONCLUSIONS : Within our cohort, the data suggested that wild-type BRAFV600E oncogene in thyroid primary tumor does not rule out its mutation in lymph node metastasis, and vice versa.

Keywords

BRAFV600E,Lymph node metastasis,PTC,