Metabolic circuits and gene regulators in polyhydroxyalkanoate producing organisms: Intervention strategies for enhanced production.

Affiliation

Sindhu R(1), Madhavan A(2), Arun KB(2), Pugazhendhi A(3), Reshmy R(4), Awasthi MK(5), Sirohi R(6), Tarafdar A(7), Pandey A(8), Binod P(9).
Author information:
(1)Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology
(CSIR-NIIST), Trivandrum 695 019, Kerala, India.
(2)Rajiv Gandhi Centre for Biotechnology, Trivandrum 695 014, Kerala, India.
(3)Innovative Green Product Synthesis and Renewable Environment Development Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Viet Nam.
(4)Post Graduate and Research Department of Chemistry, Bishop Moore College, Mavelikara 690 110, Kerala, India.
(5)College of Natural Resources and Environment, Northwest A & F University, Yangling, Shaanxi Province 712100, PR China.
(6)Department of Post Harvest Process and Food Engineering, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263 145, India.
(7)Divison of Livestock Production and Management, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243 122, Uttar Pradesh, India.
(8)Centre for Innovation and Translational Research, CSIR- Indian Institute for Toxicology Research
(CSIR-IITR), 31 MG Marg, Lucknow 226 001, India.
(9)Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology
(CSIR-NIIST), Trivandrum 695 019, Kerala, India. Electronic address: [Email]

Abstract

Worldwide worries upsurge concerning environmental pollutions triggered by the accumulation of plastic wastes. Biopolymers are promising candidates for resolving these difficulties by replacing non-biodegradable plastics. Among biopolymers, polyhydroxyalkanoates (PHAs), are natural polymers that are synthesized and accumulated in a range of microorganisms, are considered as promising biopolymers since they have biocompatibility, biodegradability, and other physico-chemical properties comparable to those of synthetic plastics. Consequently, considerable research have been attempted to advance a better understanding of mechanisms related to the metabolic synthesis and characteristics of PHAs and to develop native and recombinant microorganisms that can proficiently produce PHAs comprising desired monomers with high titer and productivity for industrial applications. Recent developments in metabolic engineering and synthetic biology applied to enhance PHA synthesis include, promoter engineering, ribosome-binding site (RBS) engineering, development of synthetic constructs etc. This review gives a brief overview of metabolic routes and regulators of PHA production and its intervention strategies.