Metabolic response of soybean leaves induced by short-term exposure of ozone.

Affiliation

Zhang X(1), Zhang X(2), Wang T(3), Li C(4).
Author information:
(1)State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, the Chinese Academy of Sciences, Beijing, China.
(2)Institute for Interdisciplinary Information Sciences, Tsinghua University, Beijing, China.
(3)State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, the Chinese Academy of Sciences, Beijing, China; College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China.
(4)State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, the Chinese Academy of Sciences, Beijing, China. Electronic address: [Email]

Abstract

The ever-increasing ozone (O3) concentration has led to reduced production and altered quality of soybean. Abundant reports have explored the damage mechanisms of O3 on soybean. However, how the elevated O3 affects metabolite profiling of soybean remains to be poorly understood. Here, we compare the metabolic profile of soybean leaves under charcoal filtered air (CF, <20 ppb) and short-term elevated O3 concentration (EO, 100 ppb). High level of O3 affects metabolites for the tricarbonic acid (TCA) cycle, reactive oxygen species, cell wall composition and amino acids. Significantly, jasmonic acid-related metabolite promoting stomata closure is highly induced with 125-fold change. Furthermore, O3 fumigation alters the expression of genes contributing to the biosynthesis of certain metabolites in TCA cycle. Together, these findings identify a wide range of changed metabolites in response to O3 pollution. Our results pave the way for the genetic improvement of soybean to adapt to O3 pollution to maintain stable yields.