Molecular detection of human papillomavirus-16 among Sudanese patients diagnosed with squamous cell carcinoma and salivary gland carcinoma.

Affiliation

Mohamed FE(1), Aldayem LN(1), Hemaida MA(1), Siddig O(2), Osman ZH(3), Shafig IR(4), Salih MAM(5)(6), Muneer MS(7)(8)(9), Hassan R(10), Ahmed ES(10), Hassan LA(10), Bakheet OEH(10), Edris AMM(1)(11), Ahmed A(12), Mohamed NS(13), Siddig EE(10)(14).
Author information:
(1)Department of Histopathology and Cytology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan.
(2)Department of Oral Pathology, Faculty of Dentistry, University of Khartoum, Khartoum, Sudan.
(3)Department of Microbiology, Faculty of Medical Laboratory Sciences, Sudan International University, Khartoum, Sudan.
(4)Department of Oral Pathology, Faculty of Dentistry, Ibn Sina University, Khartoum, Sudan.
(5)Department of Parasitology and Medical Entomology, Faculty of Medical Laboratory Sciences, Nile University, Khartoum, Sudan.
(6)Department of Clinical Chemistry, Faculty of Medical Laboratory Sciences, Karary University, Khartoum, Sudan.
(7)Department of Neurology, Mayo Clinic, Jacksonville, FL, USA.
(8)Department of Radiology, Mayo Clinic, Jacksonville, FL, USA.
(9)Faculty of Medicine, University of Khartoum, Khartoum, Sudan.
(10)Mycetoma Research Center, University of Khartoum, Khartoum, Sudan.
(11)Department of Histopathology and Cytology, Faculty of Applied Medical Sciences, University of Bisha, Bisha, Kingdom of Saudi Arabia.
(12)Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan.
(13)Department of Parasitology and Medical Entomology, Faculty of Medical Laboratory Sciences, Nile University, Khartoum, Sudan. [Email]
(14)Faculty of Medicine, Nile University, Khartoum, Sudan.

Abstract

OBJECTIVE: Human papillomavirus (HPV) gained momentum as a potential etiological factor for many types of cancers. Therefore, the aim of this study was to assess the prevalence of HPV-16 infection among Sudanese patients diagnosed with Squamous Cell Carcinoma (SCC) and Salivary Gland Carcinoma. A descriptive, hospital-based study was conducted. 150 formalin-fixed paraffin-embedded blocks were collected. RESULTS: The study population included a total of 150 patients aged between 18 to 87 years with a mean age of 48.8 ± 11.9 years. Based on gender, females constituted 46.7% while males constituted 53.3%. The 150 patients were classified into 40 (26.0%) esophageal, 30 (20.0%) nasopharyngeal, 18 (12.0%) conjunctival, 18 (12.0%) tongue 12 (8.0%) laryngeal, 8 (5.3%) lip, 6 (4.0%) oropharyngeal, 6 (4.0%) mucoepidermoid, and 6 (4.0%) adenoid cystic, and 6 (4.0%) myoepithelial carcinomas. Odds ratio for male and female diagnosed with carcinoma was 1.025 [0.439-2.394, 95% CI]. Molecular detection of HPV-16 revealed a prevalence of 26 (17.3%) patients were positive for HPV-16. According to cancer diagnosis, esophageal SCC patients showed a high proportion of HPV-16; 14/40 (35.0%). A statistically significant difference was seen for the distribution of HPV-16 positive patients based on cancer diagnosis, P value 0.001.