Monitoring mitochondrial membrane potential by FRET: Development of fluorescent probes enabling ΔΨm-Dependent subcellular migration.


Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Jinan, Shandong, 250022, PR China. Electronic address: [Email]


Mitochondrial membrane potential (ΔΨm) is a significant physiological parameter essential for many vital biological processes. In this work, two fluorescent probes enabling the subcellular migration have been rationally designed and synthesized, for the ratiometric visualization of ΔΨm via Förster resonance energy transfer (FRET) mechanism. Both the green-emitting G-1 and red-emissive MTR-1 target mitochondria in live cells, and give weak green emission and strong red emission owning to FRET process. With the loss of ΔΨm, G-1 migrates into membranous organelles, and MTR-1 moves to bind to intracellular RNA. FRET is blocked due to the separation of the two probes, and cells show strong green emission and weak red fluorescence. Consequently, the loss of ΔΨm induced by CCCP was successfully visualized in ratiometric manner, and the cell damage caused by H2O2 was monitored. We expect that the two probes can serve as validate tools in investigate ΔΨm, apoptosis, and relative areas.


FRET,Fluorescent probe,Mitochondrial membrane potential,RNA,

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