OBJECTIVE : Resistance exercise impairs endothelial function acutely. Therefore, it becomes important to devise an effective strategy for preventing acute endothelial dysfunction after resistance exercise. Herein, we tested the hypothesis that resistance exercise-induced temporal endothelial dysfunction is prevented by prior cycling. METHODS : Twelve young healthy subjects completed two randomized experimental trials: (1) resistance exercise only trial (RE trial), (2) resistance exercise with prior cycling trial (C + RE trial). Following baseline brachial artery flow-mediated dilation (FMD), the subjects maintained the supine position for 45 min in the RE trial; the subjects performed a 45 min of cycling (67.0 ± 1.7% HRmax) in the C + RE trial. After 45 min of resting or cycling, the subjects performed resistance exercise (69.7 ± 4.0 kg) at the same time points. Following the resistance exercise, they were asked to rest in the supine position for 60 min. Then FMD were repeated at 10, 30 and 60 min after the resistance exercise in both trials. RESULTS : The increased blood flow and shear rate after resistance exercise did not differ between trials, and these changes disappeared following resting in the supine position for 60 min. There was no significant interaction in %FMD responses. Both trials caused impairment in %FMD after the resistance exercise, and statistical significance was observed at 30 and 60 min after resistance exercise in the RE trial. CONCLUSIONS : The present study revealed that cycling for 45 min prior to resistance exercise was not sufficient to prevent the acute endothelial dysfunction after resistance exercise.