Palanisamy KM(1), Paramasivam P(1), Maniam GP(2), Rahim MHA(2), Govindan N(2), Chisti Y(3). Author information:
(1)Algae Culture Collection Center & Laboratory, Faculty of Industrial Sciences
& Technology, Universiti Malaysia Pahang, Lebuhraya Tun, Razak, 26300 Gambang,
Kuantan, Pahang, Malaysia.
(2)Algae Culture Collection Center & Laboratory, Faculty of Industrial Sciences
& Technology, Universiti Malaysia Pahang, Lebuhraya Tun, Razak, 26300 Gambang,
Kuantan, Pahang, Malaysia; Earth Resources & Sustainability Centre, Universiti
Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia.
(3)School of Engineering, Massey University, Private Bag 11 222, Palmerston
North, New Zealand. Electronic address: [Email]
Biomass and lipid production by the marine diatom Chaetoceros affinis were characterized under continuous light with aeration. Media based on palm oil mill effluent (POME; 10, 20 and 30 % v/v in distilled water) were used together with a standard control medium. The maximum biomass concentration on day 12 of batch cultures in control medium was 821 ± 71 mg L-1. Under identical conditions, in the best POME medium (20 % POME v/v in distilled water with other inorganic components), the biomass concentration was reduced by ∼11 % to 734 ± 66 mg L-1. The lipid content of the biomass grown in the control medium was 50.8 ± 4.5 % by dry weight, but was a little lower (48.9 ± 4.1 % by dry wt) in the above specified best POME medium. In the best POME medium, oleic acid was the major fatty acid (72.3 ± 5.2 % by weight) in the total lipids extracted from the biomass and monounsaturated fatty acids were the main type of fatty acids (74.6 ± 5.2 %). POME levels of >20 % in the medium suppressed both biomass and lipid production relative to the medium with 20 % POME.
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