Russula alutacea Fr. polysaccharide ameliorates inflammation in both RAW264.7 and zebrafish (Danio rerio) larvae.

Affiliation

State Key Laboratory of Silicon Materials, School of Materials Science and Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China. Electronic address: [Email]

Abstract

Russula alutacea Fr. (RaF) is a tasty mushroom with high nutritional value and have been regarded as food by local people for a long time. However, few researches have focused on the polysaccharide in RaF. In this study, a purified polysaccharide (Rap-1) was isolated with an average molecular weight of 1029.7 kDa. In vitro, Rap-1 significantly suppressed cell morphological changes and inhibited the production of nitric oxide (NO) in LPS-induced RAW 264.7 cells extracellularly and intracellularly. Besides, Rap-1 also down-regulated the expression of nuclear factor kappa-B (NF-κB), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) contents. Rap-1 ameliorated the oxidative stress and mitochondrial dysfunction caused by LPS via MAPKs signaling pathways in macrophages. In vivo, Rap-1 decreased the ROS and O2- levels and recovered the heart in zebrafish (Danio rerio) larvae induced by LPS, These results together suggested that Rap-1 could be a potential functional resource to protect against inflammatory and oxidative damage.

Keywords

Inflammation,Russula alutacea Fr.,Zebrafish (Danio rerio) larvae,

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