SERS-Based Colloidal Aptasensors for Quantitative Determination of Influenza Virus.

Affiliation

Gribanyov D(1), Zhdanov G(2), Olenin A(2), Lisichkin G(2), Gambaryan A(3), Kukushkin V(1), Zavyalova E(2).
Author information:
(1)Institute of Solid State Physics of Russian Academy of Science, 142432 Chernogolovka, Russia.
(2)Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia.
(3)Chumakov Federal Scientific Center for Research and Development of Immune and Biological Products RAS, 108819 Moscow, Russia.

Abstract

Development of sensitive techniques for rapid detection of viruses is on a high demand. Surface-enhanced Raman spectroscopy (SERS) is an appropriate tool for new techniques due to its high sensitivity. DNA aptamers are short structured oligonucleotides that can provide specificity for SERS biosensors. Existing SERS-based aptasensors for rapid virus detection had several disadvantages. Some of them lacked possibility of quantitative determination, while others had sophisticated and expensive implementation. In this paper, we provide a new approach that combines rapid specific detection and the possibility of quantitative determination of viruses using the example of influenza A virus.