Selection, purification, and evaluation of acarbose-an α-glucosidase inhibitor from Actinoplanes sp.

Affiliation

Tuyen DT(1), Yew GY(2), Cuong NT(3), Hoang LT(3), Yen HT(3), Hong Thao PT(3), Thao NT(3), Sy le Thanh N(3), Hien Trang NT(3), Trung NT(4), Afridi R(5), Mai Anh DT(6), Show PL(7).
Author information:
(1)Institute of Biotechnology, Vietnam Academy of Science and Technology 18 Hoang Quoc Viet Road, Caugiay Distr., 100000, Hanoi, Viet Nam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Viet Nam. Electronic address: [Email]
(2)Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, 43500, Semenyih, Selangor Darul Ehsan, Malaysia.
(3)Institute of Biotechnology, Vietnam Academy of Science and Technology 18 Hoang Quoc Viet Road, Caugiay Distr., 100000, Hanoi, Viet Nam.
(4)Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Viet Nam.
(5)KyungPook National University, Daegu, South Korea.
(6)Department of Biochemistry, Hanoi University of Pharmacy, Viet Nam.
(7)Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, 43500, Semenyih, Selangor Darul Ehsan, Malaysia. Electronic address: [Email]

Abstract

Actinoplanes sp. A1094 strain had been selected for its high production of acarbose from 20 different strains of Actinoplanes sp. can be found in wild. The content for glucosidase inhibitor of acarbose concentration was recorded at 1.12 g/L. The conducted analysis of 16S rRNA sequence of Actinoplanes sp. A1094 showed 99% similar identity to the corresponding sequence of Actinoplanes hulinensis. Acarbose was purified from Actinoplanes hulinensis 1094 with a yield of 8.48%, purity of 98% and further identified by LC/MS and NMR methods (C25H43NO18; m/z: 645.6 g/mol). The purified acarbose was used to evaluate the hypoglycemia in streptozotocin (STZ)-induced diabetic mice model. The purified acarbose reduced postprandial blood glucose level in comparison with Glucobay® as medication for control type 2 diabetes in a combination therapy. Notably, the outcomes of native acarbose on fasting blood glucose levels in mice resemble akin to the commercial product and the acarbose accumulating fermentation and metabolic engineering from the cell gene in which would reduce in production cost. Therefore, acarbose from Actinoplanes hulinensis 1094 could be potentially used to make products for the treatment of type II diabetes.