Streptomyces scabichelini sp. nov., isolated from soil.

Affiliation

Gencbay T(1), Saygin H(2), Guven K(3), Topkara AR(1), Saricaoglu S(4), Sahin N(2), Isik K(1).
Author information:
(1)Department of Biology, Faculty of Science and Arts, Ondokuz Mayis University, Samsun 55139, Turkey.
(2)Department of Molecular Biology and Genetics, Faculty of Sciences and Arts, Ondokuz Mayis University, Samsun 55139, Turkey.
(3)Department of Biology, Faculty of Science, Eskisehir Technical University, Eskisehir 26555, Turkey.
(4)Department of Therapy and Rehabilitation, Health Services Vocational College, Kirsehir Ahi Evran University, Kirsehir 44200, Turkey.

Abstract

A novel actinomycete, designated strain HC44T, was isolated from a soil sample collected from Hacibektaş, Turkey, and characterized using a polyphasic approach. The strain had morphological characteristics and chemotaxonomic properties identical to those of members of the genus Streptomyces. Phylogenetic analyses based on 16S rRNA gene sequence comparisons revealed that HC44T clustered with members of the genus Streptomyces and the highest 16S rRNA gene sequence similarity values were obtained with Streptomyces vastus NBRC 13094T (97.6 %) and Streptomyces kalpinensis TRM 46509T (96.9 %). Multi-locus sequence analysis (MLSA) based on five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) showed that the MLSA evolutionary distance value was 0.043 between strain HC44T and S. vastus NBRC 13094T. Whole-cell hydrolysates contained ll-diaminopimelic acid, glucose, mannose and ribose. The predominant menaquinones were MK-9(H6) and MK-9(H8). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The DNA G+C content of the draft genome sequence, consisting of 11.2 Mbp, was 69.8 mol%. On the basis of polyphasic taxonomic evidence, strain HC44T represents a novel species of the genus Streptomyces, for which the name Streptomyces scabichelini sp. nov. is proposed. The type strain is HC44T (=DSM 106874T=KCTC 39872T).