Substrate-free and label-free electrocatalysis-assisted biosensor for sensitive detection of microRNA in lung cancer cells.

Affiliation

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China. [Email]

Abstract

We develop a substrate-free and label-free electrocatalysis-assisted biosensor for sensitive detection of microRNA using the iron-embedded nitrogen-rich carbon nanotubes (FeCN) as the catalytic elements. This biosensor exhibits excellent selectivity and high sensitivity with a detection limit of 8.53 × 10-16 M and a large dynamic range of 6 orders of magnitude. It can be further applied for accurate quantification of microRNA in lung cancer cells.