Tandem Quantification of Multiple Carbohydrates in Saliva Using Surface-Enhanced Raman Spectroscopy.


Lin D(1), Yang SW(2), Hsieh CL(2), Hsu KJ(2), Gong T(3), Wu Q(1), Qiu S(4), Feng S(1), Kong KV(2).
Author information:
(1)Key Laboratory of OptoElectronic Science and Technology for Medicine, Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Digital Fujian Internet-of-Things Laboratory of Environment Monitoring, Fujian Normal University, Fuzhou 350007, China.
(2)Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.
(3)State Key Laboratory of Electronic Thin Films and Integrated Devices, School of Electronic Science and Engineering
(National Exemplary School of Microelectronics), University of Electronic Science and Technology of China, Chengdu 610054, China.
(4)Department of Radiation Oncology, Fujian Provincial Key Laboratory of Translational Cancer Medicine, Fujian Medical University Cancer Hospital & Fujian Cancer Hospital, Fuzhou 350014, China.


The detection of carbohydrates in human body fluids is critical for disease diagnosis and healthy monitoring. Despite recent advances in glucose sensing, multiplex detection of different carbohydrates within a single assay that is capable of efficiently providing richer health information remains challenging. Herein, we report a versatile surface-enhanced Raman spectroscopy-based platform for the quantitative detection of monosaccharides (glucose, fructose, and galactose) in one test using a displace-and-trap mechanism. Moreover, due to the use of multiple optical interference-free (1800-2200 cm-1) signal-independent Raman probes, the detection range of this platform (0.125-7 mg/dL) perfectly covers physiological concentrations, enabling the quantitative detection of glucose and galactose in clinical human saliva samples. This work provides a noninvasive and high-efficiency potential tool for the screening of clinical diabetes and other carbohydrate-related diseases.