The dynamic changes of glycogen molecular structure in Escherichia coli BL21(DE3).

Affiliation

Wang M(1), Liu Q(2), Li F(3), Tang J(4), Xiong X(3), Yang Y(5), Ju P(5), Wang Z(6), Gilbert RG(6), Wang L(7).
Author information:
(1)Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, Xuzhou, 221000, Jiangsu Province, China; Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, Xuzhou, 221000, Jiangsu Province, China.
(2)Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, Xuzhou, 221000, Jiangsu Province, China; Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, Xuzhou, 221000, Jiangsu Province, China.
(3)School of Laboratory Medicine, Xuzhou Medical University, Xuzhou, Jiangsu, 221000, China; Department of Laboratory Medicine, Huaiyin Hospital, Huai'an, Jiangsu, 223000, China.
(4)Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, Xuzhou, 221000, Jiangsu Province, China.
(5)School of Life Science, Xuzhou Medical University, Xuzhou, Jiangsu, 221000, China.
(6)School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, 4072, Australia; Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, Queensland, 4072, Australia; Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture, Yangzhou University, Yangzhou, 225009, Jiangsu Province, China.
(7)Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, Xuzhou, 221000, Jiangsu Province, China; Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, Xuzhou, 221000, Jiangsu Province, China. Electronic address: [Email]

Abstract

Diurnal alteration of glycogen molecular structure has been identified in healthy mice. Recently, both fragile (disintegration in dimethyl sulfoxide) and stable (not disintegrating in DMSO) glycogen particles were found in Escherichia coli. However, how glycogen structure changes dynamically in E. coli is not clear. The question examined here is whether fragile, stable glycogen α particles occur in bacteria, following a similar pattern as in mice. In this study, we examine the dynamic changes of glycogen molecular structure over 24-h in E. coli BL21(DE3), using transmission electron microscopy, size exclusion chromatography and fluorophore-assisted carbohydrate electrophoresis at representative time points. It was found that glycogen structure was mainly fragile at the synthesis stage and largely stable during the degradation stage. qRT-PCR results indicated that balance of anabolic and catabolic gene expression levels in glycogen metabolism could be a key factor affecting the fragility of glycogen α particles in bacteria.