The effect of recycling culture medium after harvesting of Chlorella vulgaris biomass by flocculating bacteria on microalgal growth and the functionary mechanism.


College of Life Sciences, Henan Normal University, Xinxiang 453007, China; Advanced Environmental Biotechnology Center, Nanyang Environment and Water Research Institute, Nanyang Technological University, Singapore 637141, Singapore. Electronic address: [Email]


Microalgal cultivation and harvesting are two main factors that restrict the development of microalgae. To help solve these two questions, flocculating bacteria and recycling culture medium were investigated in this study. Proteins in bacterial cells of Bacillus sp. y3 and y6 exhibited direct flocculation activities on Chlorella vulgaris cells. The recycling culture media after harvest of microalgal biomass by strain y3 and y6 could promote C. vulgaris growth. The determination of bacterial community confirmed that strain Bw in C. vulgaris phycosphere was the bacterial species that played the major promotion effect on microalgal growth. The functionary mechanism of strain y3 and y6 on microalgal growth was determined that they can influence C. vulgaris growth by changing the concentration of strain Bw to regulate algal ROS and antioxidant system response. The present study can provide effective research ideas and the application potential for the cultivation and harvesting of microalgae.


Bacillus sp. y3 and y6,Flocculation activity,Functionary mechanism,Microalgal cultivation and harvesting,Recycling culture medium,

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